Remote Ischemic Preconditioning of the Femoral Artery and Vein does not Protect against Renal Ischemia/Reperfusion-Induced Injury in Anesthetized Mice

Male C57BL/6 mice at 5 weeks of age were purchased from CLEA (Tokyo, Japan). The mice were uninephrectomized 10 days prior to I/R and RIPC. We examined seven different RIPC protocols to determine whether RIPC induces renal protection against renal I/R injury in either pentobarbital (Figure 1 and 2) or isoflurane (Figure 3) anesthetized mice. All experimental procedures were performed according to the guidelines for the care and use of animals established by Kagawa University.